16 DAP destructive sampling collections were done from 8/10/15 through 8/20/15. Details of samples collected on each date can be found in /2015_Field_Files/phenotype_files/distructive_sampling_pollination_notes_080415.xlsx

For each genotype, three replicates were harvested that each consisted of tissue from three plants. Endosperm and embryo tissue from 50 kernels per ear (150 total for each replicate) were pooled to make up a single replicate. When only two ears were available, 75 kernels from each ear were dissected and pooled to make 150 total for the replicate. Internode tissue from the internode above the ear bearing node was also harvested from the same three plants.