Monday, June 29, 2015

Rep1 of the BiomAP inbred/hybrid greenhouse experiment was measured and harvested on 6/26/15 from 8:00-2:30. Samples were placed in the oven at 85F from 6/26/15-6/26/15.

Rep2 of the BiomAP inbred/hybrid greenhouse experiment was measured and harvested on 6/29/15 from 8:00-1:00.
Placed Biomap Greenhouse planting samples in Drier #1 (140°) shelf #2 at 2:00pm on June 29, 2015.

Thursday, June 25, 2015

5th leaves were punched for the BiomAP inbred/hybrid trial in X5 on 06/22/15.

Wednesday, June 24, 2015

Updated experimental design files with information on bad sources found by looking for albinos in the field.

Bad sources:
LH220Ht SP14:11302 W8555 SP14:11460 PHN46 SP14:11383

Files affected:
GxE_NIL_HOF_experimental_design_with_reps_FINAL.xlsx (PHN46)
Yield_Component_FINAL.xlsx (LH220Ht, W8555, PHN46)
X5_field1_short_rows_late_FINAL.xlsx (PHN46 in HOF Diallel and Inbred Increase)
X6_field2_short_rows_early_FINAL.xlsx (PHN46 in Plants for Destructive Sampling)
X7_field3_long_rows_early_FINAL.xlsx (PHN46 in Plants for Distructive Tissue Sampling)

A list of all the affected plots is available in 2015_FieldFiles/phenotype_files/ALL_bad_sources.xlsx.

Monday, June 22, 2015

The 6 DAS greenhouse destructive sampling was done from 9:30-11:30 on 6/22/15.

For each genotype (DK3IIH6, LH185, LH82, MoG, PH207, PHJ89, PHK56, PHK76, PHN46, PHP02, W22, W606S, B73, PHJR5, PHK35) 3 replicates were harvested. Each replicated contained three plants at the 6 DAS developmental stage with the exception of LH185 that only had two plants per replicate.

There were two plants (one from PHN46 rep2 and one from PHK76 rep2) that looked stressed and were not harvested. These plants had white, yellow, and pink coloring in the leaves.

These samples are al in labeled bags in the fourth shelf of the -80 freezer.
Rep2 of the BiomAP inbred/hybrid greenhouse experiment for measuring seedling root length, above ground height, root weight, and above ground weight was planted on 6/22/15. The greenhouse conditions are 16h day/8 h night with supplemental light only as needed and temperatures are at 27C day/24C night.

Friday, June 19, 2015

5th leaves were punches for the BiomAP inbred/hybrid trial in X6 on 06/15/15.
Rep1 of the BiomAP inbred/hybrid greenhouse experiment for measuring seedling root length, above ground height, root weight, and above ground weight was planted on 6/19/15. The greenhouse conditions are 16h day/8 h night with supplemental light only as needed and temperatures are at 27C day/24C night.

Thursday, June 18, 2015

X5, X6, and X7 were all sprayed with atrazine on 6/8/15 to help with emerging grass issue. The fields were rechecked on 6/17/15 and look good. 

GPS Coordinates for GxE 2015 St Paul trail (IGXE)

NW corner 44.994383, -93.17476
SW corner 44.994343, -93.174755
NE corner 44.994376, -93.174385
SE corner 44.994381, -93.174334

Weather station 44.994307, -93.175327
Stand counts on all X5 plots except HOF/NIL were collected on 6/12/15 and are available here.

Tuesday, June 16, 2015

The inbreds for destructive sampling in the greenhouse at 6 DAS were soaked overnight on the night of 6/15/15 and planted on 6/16/15. The greenhouse conditions are 16h day/8 h night with supplemental light only as needed and temperatures are at 27C day/24C night.

The final experimental file was updated on 6/16/15 to change the boarder pots from B73 to Mo17.

Thursday, June 11, 2015

Stand counts on all X6 plots except HOF/NIL were collected on 6/4/15 and are available here.

Tuesday, June 9, 2015

The first phenotyping by blueriver was done on 6/8/15 and including the entire front section of X6.