Friday, August 28, 2015
Monday, August 24, 2015
16 DAP destructive sampling collections were done from 8/10/15 through 8/20/15. Details of samples collected on each date can be found in /2015_Field_Files/phenotype_files/distructive_sampling_pollination_notes_080415.xlsx
For each genotype, three replicates were harvested that each consisted of tissue from three plants. Endosperm and embryo tissue from 50 kernels per ear (150 total for each replicate) were pooled to make up a single replicate. When only two ears were available, 75 kernels from each ear were dissected and pooled to make 150 total for the replicate. Internode tissue from the internode above the ear bearing node was also harvested from the same three plants.
For each genotype, three replicates were harvested that each consisted of tissue from three plants. Endosperm and embryo tissue from 50 kernels per ear (150 total for each replicate) were pooled to make up a single replicate. When only two ears were available, 75 kernels from each ear were dissected and pooled to make 150 total for the replicate. Internode tissue from the internode above the ear bearing node was also harvested from the same three plants.
Wednesday, August 5, 2015
Monday, August 3, 2015
The fourth round of R1 field destructive sampling was done from 2:00-2:30 on 08/03/15.
The final three genotypes were ready today (LH185, MoG, PHJR5). For each genotype 3 replicates were harvested with the exception of PHJR5 which only had 1 replicate that needed to be harvested. Each replicate contained three plants at the R1 developmental stage. The tassel (anthers) were harvested. LH185 was harvested from a block in X5 (SP15:10146-10155), MoG was harvested from the block in X6 (SP15:20673-20720), and PHJR5 was harvested from the yield component experiment in X5.
These samples are all in labeled bags in the -80 freezer.
The final three genotypes were ready today (LH185, MoG, PHJR5). For each genotype 3 replicates were harvested with the exception of PHJR5 which only had 1 replicate that needed to be harvested. Each replicate contained three plants at the R1 developmental stage. The tassel (anthers) were harvested. LH185 was harvested from a block in X5 (SP15:10146-10155), MoG was harvested from the block in X6 (SP15:20673-20720), and PHJR5 was harvested from the yield component experiment in X5.
These samples are all in labeled bags in the -80 freezer.
The third round of R1 field destructive sampling was done from 2:30-3:00 on 07/30/15.
Six of the genotypes were ready today (W22, B73, PHK76, PHK56, PHK35, PHJR5). For each genotype 3 replicates were harvested with the exception of PHJR5 which only had 2 replicates that were ready. Each replicate contained three plants at the R1 developmental stage. The tassel (anthers) were harvested. W22 was harvested from the block in X6 (SP15:20673-20720), B73 was harvested from the extra females range in the back range of X6, PHK76 and PHK56 were harvested from the second (delay) block in X7 (SP15:30169-30192), PHK35 was harvested from the yield component experiment in X6, PHJR5 was harvested from the yield component experiment in X5.
These samples are all in labeled bags in the -80 freezer.
NOTE: I had a note that we collected PHK56 in the second harvest, but we believe this was really PHP02 and that the notes that I took were incorrect.
The following samples still need to be collected:
LH185
MoG
PHJR5 - rep3
Six of the genotypes were ready today (W22, B73, PHK76, PHK56, PHK35, PHJR5). For each genotype 3 replicates were harvested with the exception of PHJR5 which only had 2 replicates that were ready. Each replicate contained three plants at the R1 developmental stage. The tassel (anthers) were harvested. W22 was harvested from the block in X6 (SP15:20673-20720), B73 was harvested from the extra females range in the back range of X6, PHK76 and PHK56 were harvested from the second (delay) block in X7 (SP15:30169-30192), PHK35 was harvested from the yield component experiment in X6, PHJR5 was harvested from the yield component experiment in X5.
These samples are all in labeled bags in the -80 freezer.
NOTE: I had a note that we collected PHK56 in the second harvest, but we believe this was really PHP02 and that the notes that I took were incorrect.
The following samples still need to be collected:
LH185
MoG
PHJR5 - rep3
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