Tuesday, July 28, 2015

The fourth round of V15 field destructive sampling was done from 1:30-3:00 on 07/28/15.

The final genotype was ready today (MoG). Three replicates were harvested. Each replicated contained three plants at the ~V15 developmental stage (Note: the bags say V18 as we thought this would be the stage when the plants would be ready, but on average they are at V15). The immature tassel and immature ear were harvested. MoG was collected from the block in X6 (SP15:20703).

These samples are all in labeled bags in the -80 freezer.

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The second round of R1 field destructive sampling was done from 1:30-3:00 on 07/28/15.

Three of the genotypes were ready today (PHK56, PHN46, and W606S). For each genotype 3 replicates were harvested. Each replicate contained three plants at the R1 developmental stage. The tassel (anthers) were harvested. PHK56 was harvested from the first block in X7 (SP15:30145-SP15:30167) and PHN46 and W606S were harvested from the block in X6 (SP15:20673-20720).

These samples are all in labeled bags in the -80 freezer.

The following samples still need to be collected:
LH185
MoG
PHK76
PHP02
W22
B73
PHJR5
PHK35

Thursday, July 23, 2015

The third round of V15 field destructive sampling was done from 1:30-3:00 on 07/23/15.

Four of the genotypes were ready today (LH185, PHK76, PHK35, and PHJR5). For each genotype 3 replicates were harvested. Each replicated contained three plants at the ~V15 developmental stage (Note: the bags say V18 as we thought this would be the stage when the plants would be ready, but on average they are at V15). The immature tassel and immature ear were harvested. LH185 and PHK76 were collected from the second (delay) block in X7 (SP15:30169-30192), PHK35 was harvested from the yield component trial in X6 from rep 1 (YC15:1324) and PHJR5 was harvested from the yield component trial in X5 from rep 4 (YC15:4240).

These samples are all in labeled bags in the -80 freezer.

The genotypes that still need to be sampled include:
MoG

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The first round of R1 field destructive sampling was done from 3:00-3:00 on 07/23/15.

Four of the genotypes were ready today (LH82, DK3IIH6, PH207, and PHJ89). For each genotype 3 replicates were harvested. Each replicate contained three plants at the R1 developmental stage. The tassel (anthers) were harvested. Everything was harvested from the first block in X7 (SP15:30145-SP15:30167).

These samples are all in labeled bags in the -80 freezer.

Monday, July 20, 2015

The second round of V15 field destructive sampling was done from 1:30-3:00 on 07/20/15.

Six of the genotypes were ready today (DK3IIH6, LH82, PHK56, PHN46, W606, B73). For each genotype 3 replicates were harvested. Each replicated contained three plants at the ~V15 developmental stage (Note: the bags say V18 as we thought this would be the stage when the plants would be ready, but on average they are at V15). The immature tassel and immature ear were harvested. All samples were collected from the first block in X7 (SP15:30145-30167) except for B73 that was harvested from SP15:20612.

These samples are al in labeled bags in the -80 freezer.

The genotypes that still need to be sampled include:
LH185
MoG
PHK76
PHJR5
PHK35

Wednesday, July 15, 2015

The first round of V15 field destructive sampling was done from 1:00-2:30 on 07/15/15.

Four of the genotypes were ready today (PH207, PHJ89, PHP02, and W22 (a little bit early, but still sampled)). For each genotype 3 replicates were harvested. Each replicated contained three plants at the V15 developmental stage (Note: the bags say V18 as we thought this would be the stage when the plants would be ready, but on average they are at V15). The immature tassel and immature ear were harvested. All samples were collected from the first block in X7 (SP15:30145-30167).

These samples are al in labeled bags in the -80 freezer.

Tuesday, July 14, 2015

Collars were put on between the 10th and 11th leaves in the hybrid plots on 07/08/15 and the inbred plots on 07/14/15 of the second St Paul planting of the biomap experiment.

Wednesday, July 8, 2015

Waseca Notes 070715

Notes on issues from trip to Waseca on 070715. Low density plots in the HN15 trial were thinned on 070715.

Maps of the issues in this post are available in 2015_Field_Files/pictures/Waseca_070715/Waseca_Field_Damage_070715.xlsx

Photos available at 2015_Field_Files/pictures/Waseca_070715/


HGXE trail had no issues aside from a clogged rain catcher on the weather station. Plants were V9-V10. The station was cleared of debris and confirmed as working.

Planting 1 had some known issues that occurred during planting. Plots below had no plants growing in them, and no sign of ever having any emerged plants:
BM15:5063
BM15:5058
BM15:5045
BM15:5044
BM15:5042
5 additional BxM Border rows in the front right corner of the Biomap trial

Planting 1 of the YC trial was at V9-V10. The back of this trial is at a low point and suffered water damage. Plots with visible water damage:
YC15:6396
YC15:6397
YC15:6398
YC15:6399
YC15:6400
YC15:6401
YC15:6402
YC15:6403
YC15:6446
YC15:6447
YC15:6448
YC15:6449
YC15:6450
YC15:6451
YC15:6452
YC15:6453
YC15:6454
YC15:6455
YC15:6456
YC15:6457
YC15:6458
YC15:6459
YC15:6460
YC15:6461
YC15:6462
YC15:6463
YC15:6464
YC15:6465
YC15:6466
YC15:6467
YC15:6468
YC15:6469
YC15:6470
YC15:6471
YC15:6472
YC15:6473
YC15:6474
YC15:6475
YC15:6476
YC15:6477
YC15:6478
YC15:6479
YC15:6480
Additional border rows

Planting 2 of high density HN15 hybrids was at V7-V9.
Planting 2 of low density HN15 hybrids was at V8-V9.
Planting 2 of low density HN15 inbreds was at V7-V8.

Plots in planting 2 that had visible water damage:
BM15:8004
BM15:8005
BM15:8006
BM15:8007
BM15:8008
BM15:8009
BM15:8010
BM15:8011
BM15:8012
BM15:8013
BM15:8014
BM15:8015
BM15:8016
BM15:8017
BM15:8018
BM15:8019
BM15:8020
BM15:8021
BM15:8022
BM15:8023
BM15:8024
BM15:8025
BM15:8026
BM15:8027
BM15:8028
BM15:8029
BM15:8030
HN15:110067
HN15:110068
HN15:110069
HN15:110070
HN15:110071
HN15:110072
HN15:110073
HN15:110074
HN15:110075
HN15:110076
HN15:110077
HN15:110078
HN15:110079
HN15:110080
HN15:110081
HN15:110082
HN15:110083
HN15:112067
HN15:112068
HN15:112069
HN15:112070
HN15:112071
HN15:112072
HN15:112073
HN15:112074
HN15:112075
HN15:112076
HN15:112077
HN15:112078
HN15:112079
HN15:112080
HN15:112081
HN15:112082
HN15:112083
YC15:8361
YC15:8362
YC15:8363
YC15:8364
YC15:8365
YC15:8425
YC15:8426
YC15:8427
YC15:8428
YC15:8429
YC15:8430
YC15:8431
YC15:8432
YC15:8433
YC15:8434
YC15:8435
YC15:8436
YC15:8437
YC15:8438
YC15:8439
YC15:8440
YC15:8441
YC15:8442
YC15:8443
YC15:8444
YC15:8445
YC15:8446
YC15:8447








Thursday, July 2, 2015

The V11 field destructive sampling was done from 9:00-11:30 on 07/02/15.

For each genotype (DK3IIH6, LH185, LH82, MoG, PH207, PHJ89, PHK56, PHK76, PHN46, PHP02, W22, W606S, B73, PHJR5, PHK35) 3 replicates were harvested. Each replicated contained three plants at the V11 developmental stage. The first leaf without a ligule initiated was dissected for each plant (~13 leaf) and the base (papery white tissue), light green tissue in the middle, and dark green tissue at the tip of the leaf was harvested.

All samples were collected from the first block in X7 (SP15:30145-30167) with the exception of LH185 reps 2 and 3 were harvested from X6 back (SP15:20120-20182), B73 reps 1-3 from X6 back (SP15:20610), PHJR5 reps 1-3 from X6 yield component trial (YC15:2038), and PHK35 reps 1-3 from X6 yield component trial (YC15:2329).

These samples are al in labeled bags in the -80 freezer.

Wednesday, July 1, 2015

Data from the greenhouse BiomAP planting collected on 062615 and 062915 has been QC'd and is available at Drive/2015_Field_Files/phenotype_files/GHB

GHB_freshAGmass.xlsx
GHB_freshBGmass.xlsx
GHB_looseheight.xlsx
GHB_pulledheight.xlsx
GHB_rootlength.xlsx